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DNA sequencing III : dealing with difficult templates / edited by Jan Kieleczawa.

Contributor(s): Material type: TextTextPublication details: Sudbury, Mass. : Jones and Bartlett Publishers, c2009.Description: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cmISBN:
  • 9780763742973 (alk. paper)
  • 076374297X (alk. paper)
Other title:
  • DNA sequencing three
  • DNA sequencing 3
Subject(s): DDC classification:
  • 572.86 22
Contents:
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
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Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.

Biotechnology & Bioinformatics