IUB Library OPAC
DNA sequencing III : dealing with difficult templates / edited by Jan Kieleczawa.
Material type:
TextPublication details: Sudbury, Mass. : Jones and Bartlett Publishers, c2009. Description: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cmISBN: 9780763742973 (alk. paper); 076374297X (alk. paper)Other title: DNA sequencing three | DNA sequencing 3Subject(s): DNA -- Analysis | Nucleotide sequence -- Methodology | Sequence Analysis, DNA -- methods | Base Sequence | Templates, GeneticDDC classification: 572.86 | Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds |
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Reference Stacks | Non-fiction | 572.86 D629 2009 (Browse shelf(Opens below)) | 01 | Available | 020199 |
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Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
Biotechnology & Bioinformatics