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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / Reiner Westermeier.

By: Material type: TextTextPublisher: Weinheim, Germany : Wiley-VCH, 2016Edition: Fifth editionDescription: 1 online resourceContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9783527695188
  • 3527695184
  • 9783527695164
  • 3527695168
  • 9783527695195
  • 3527695192
  • 9783527695171
  • 3527695176
Subject(s): Genre/Form: DDC classification:
  • 541/.372 23
LOC classification:
  • QP519.9.E434
Online resources:
Contents:
Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.
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Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.

Includes bibliographical references and index.

Online resource; title from PDF title page (John Wiley, viewed March 7, 2016).

Physical Science