01629nam a2200289 a 450000500170000000100090001700800410002602000310006702000280009804000180012608200150014424500880015924600250024724600210027226000610029330000620035450400510041650506880046765000190115565000380117465000370121265000190124965000240126870000210129299900170131352600090133020230823095608.000013856130616s2009    mauaf    b    001 0 eng    a9780763742973 (alk. paper)  a076374297X (alk. paper)  aDLCcBD-DhIUB00a572.8622200aDNA sequencing III :bdealing with difficult templates /cedited by Jan Kieleczawa.3 aDNA sequencing three3 aDNA sequencing 3  aSudbury, Mass. :bJones and Bartlett Publishers,cc2009.  axvi, 184 p., 8 p. of plates :bill. (some col.) ;c24 cm.  aIncludes bibliographical references and index.0 aControlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates. 0aDNAxAnalysis. 0aNucleotide sequencexMethodology.12aSequence Analysis, DNAxmethods.22aBase Sequence.22aTemplates, Genetic.1 aKieleczawa, Jan.  c13899d13899  bbtbi